Journal: Cell Reports Medicine
Article Title: A candidate loss-of-function variant in SGIP1 causes synaptic dysfunction and recessive parkinsonism
doi: 10.1016/j.xcrm.2024.101749
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Article Snippet: The following antibodies were used: guinea pig anti-EndoA (GP69) [1:2000], rabbit anti-GFP [1:1000 (Invitrogen)], mouse anti-Brp [1:50 (DSHB)], mouse anti-Syntaxin1A [1:50 (DSHB)], mouse anti-CSP [1:50 (DSHB)], mouse anti-GluRIIA [1:100 (DSHB)], mouse anti-Synaptotagmin1 [1:50 (DSHB)], rabbit anti-Synaptojanin [1:500], guinea pig anti-Vha100-1 [1:2000 (gift from Robin Hiesinger ], mouse anti-Synapsin [1:100 (DSHB)], mouse anti-Dynamin [1:500 (BD Biosciences)], rat anti-Synaptobrevin [1:1000 (gift from Hugo Bellen)], mouse anti-DLG [1:50 (DSHB)], rabbit anti-HRP [1:1000 (Jackson ImmunoResearch)], rabbit anti-TH [1:200 (Millipore)].
Techniques: Recombinant, Bradford Protein Assay, Sequencing, Cloning, Software, Functional Assay, Imaging